Objective To evaluate the quality of animal studies into acupuncture for glaucoma using SYRCLE’s risk of bias tool, ARRIVE 2. 0 guidelines, and the GSPC checklist. Methods Databases from CNKI, VIP, Wanfang, Sinomed, PubMed, Web of Science, Embase and Cochrane Library were searched to find animal research articles on acupuncture for glaucoma. Risk of bias was assessed for the included studies using the SYRCLE’ s tool, and reporting quality was evaluated using the ARRIVE 2. 0 guidelines and GSPC checklist. Statistical analysis was performed by Excel and SPSS software. Results Thirty articles met the inclusion/ exclusion criteria and were included in the final analysis. Six of the 10 items of the SYRCLE’s tool had a low-risk rate of <50%, and the non-low-risk items focused on selectivity bias, implementation bias and measurement bias. Twelve of the 22 essential sub-items of the ARRIVE 2. 0 guidelines had a low-risk rate of <50%; 9 of the 16 recommended sub-items had a low-risk rate of <50%; and 12 of the 19 subentries of the GSPC list had a low-risk rate of <50%. Randomization, blinding, ethical statements, housing and husbandry, animal care and monitoring, and protocol registration were the non-low-risk items in the ARRIVE 2. 0 guidelines and GSPC list. Conclusions The quality of the methodology and experimental reporting of animal studies into acupuncture for glaucoma are generally low, and the description of several items is not yet complete, which affects the readers’ judgment on whether the result of animal studies can be translated to clinical studies. It is advisable to further promote the use of SYRCLE’s tool and reporting guidelines for animal experiments to enhance the design, performance, and reporting of animal experiments; ensure the reproducibility of experiments and result ; and provide reliable evidence for the translation of result to the clinic.