刹车片颗粒及其主要成分硫化锑呼吸暴露对小鼠健康危害研究
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中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,国家中医药管理局人类动物模型三级实验室,北京 100021

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R-33


Experimental study on the health hazards of respiratory exposure to brake pad particles and their main component antimony sulfide in mice
Author:
  • LI Yanhong

    LI Yanhong

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Center of Comparative Medicine, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • XIU Jinghui

    XIU Jinghui

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Center of Comparative Medicine, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • ZHOU Li

    ZHOU Li

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Center of Comparative Medicine, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • GUO Jianguo

    GUO Jianguo

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Center of Comparative Medicine, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine, Beijing 100021, China
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Affiliation:

Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Center of Comparative Medicine, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine, Beijing 100021, China

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    摘要:

    目的 研究刹车制动来源颗粒物对机体的潜在危害。 方法 研磨刹车片颗粒、硫化锑颗粒,小鼠通过气管灌注暴露 1 个月后,观察肺、心脏、肝等内脏器官病理改变,同时用流式细胞术分析外周血巨噬细胞和自然调节 T 淋巴细胞(regulatory T cell,Treg)的变化。 结果 刹车片颗粒及硫化锑颗粒暴露小鼠肺泡壁外源物质沉积,血管周围出现明显的炎症细胞浸润并随着暴露浓度的增加而加重,同时可出现心脏血管炎症和微血栓以及肝细胞肿胀等病理改变;Treg 细胞减少,硫化锑组和低剂量刹车片组巨噬细胞增加,同时 M2 型巨噬细胞比例下调。 结论刹车片颗粒及其成分硫化锑颗粒暴露对小鼠肺、心脏、肝等脏器具有不同程度的毒性作用,并对免疫系统造成了影响,提示刹车来源的空气污染存在潜在健康危害。

    Abstract:

    Objective To study the potential harmful effects of brake-pad sourced particles. Methods Grind the brake pad particles or antimony sulfide particles. Mice were exposed brake pad or antimony sulfide particles by tracheal perfusion for 1 month. We observed pathological changes to the lungs, heart, and liver, and analyzed changes in peripheral blood macrophages and regulatory T cells (Tregs) via flow cytometry. Results After exposure, the deposition of foreign substances in the alveolar wall of mice was seen, with the obvious infiltration of inflammatory cells around blood vessels,which worsened with increasing particle concentration. Pathological changes, such as vascular inflammation and microthrombosis in the heart and hepatocyte swelling in the liver, were observed. Treg cells in peripheral blood decreased and macrophages increased in the antimony sulfide group and low-dose brake pad particles group, while the proportion of M2-type macrophages decreased, in the antimony sulfide group and low-dose brake pad particle group. Conclusions Exposure to brake pad particles and their component antimony sulfide has varying degrees of toxic effects on the lung,heart, and liver of mice and has an impact on the immune system, indicating the potential health hazards of brake-padderived air pollution.

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李彦红,修晶辉,周 澧,郭建国.刹车片颗粒及其主要成分硫化锑呼吸暴露对小鼠健康危害研究[J].中国比较医学杂志,2024,34(3):10~17.

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  • 收稿日期:2023-09-08
  • 在线发布日期: 2024-05-29
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