Laboratory animal science plays a crucial role in the education and training of medical students. To ensure effective teaching of laboratory animal science in Chinese medicine institutions, the principles of Chinese medicine characteristics and innovation should be incorporated. This pedagogical approach aims to enrich traditional cultural literacy in Chinese medicine and foster the students’ capacities for innovation and critical thinking. Daily teaching of laboratory animal science should encompass various educational aspects, including inheritance, ethics, migration, exploration,inspiration, and extension education. By integrating these components and emphasizing the intersections of Chinese medicine with laboratory animal science, important areas, such as animal welfare in Chinese medicine research, the application of Chinese medicine theories in animal experiments, considerations for animal models in Chinese medicine, and the selection of experimental animal species, can be addressed. Additionally, this approach guides new research directions and effectively cultivates the students’ scientific research, innovation, and practical abilities. The primary Objective of this program is to nurture scientific innovation and practical competence among students while prioritizing the superior quality of innovative education in the context of Chinese medicine. Such efforts offer a solid foundation to advance traditional medical practices and nurture a new generation of professionals dedicated to preserving traditional medicine.