+高级检索
首页 > 过刊浏览>2024年第34卷第8期 >87-93. DOI:10.3969/j.issn.1671-7856.2024.08.010
PDF HTML阅读 XML下载 导出引用 引用提醒
无菌金黄地鼠模型建立
作者:
作者单位:

中国医学科学院医学实验动物研究所,国家动物模型技术创新中心,国家卫生健康委比较医学重点实验室,国家人类疾病动物模型资源库,北京 100021

中图分类号:

R-33


Establishment of sterile golden hamster model
Author:
Affiliation:

Institute of Laboratory Animal Sciences, CAMS & PUMC, National Center of Technology Innovation for animal model,NHC Key Laboratory of Comparative Medicine, National Human Diseases Animal Model Resource Center, Beijing 100021, China

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献
    • |
  • 相似文献
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    目的 使用剖宫产净化( cesarean section purification)方法建立无菌金黄地鼠模型。 方法 供胎母鼠选择 SPF 级金黄地鼠,雌雄按 1 ∶ 1 进行交配,见栓后与雄鼠分开饲养。 代乳母鼠的合笼时间应早于供胎母鼠一周。 怀孕的金黄地鼠临产时在超净工作台内进行子宫摘除术,传入净化用隔离包内,剥离子宫获得待净化乳鼠,分别使用无菌级 ICR 小鼠和无菌级 SD 大鼠进行代乳,成功离乳后转入饲养用隔离包内。 每月对饲养用隔离包进行无菌状态检测。 结果 共实施 3 次剖宫产手术,第 1 次和第 2 次代乳失败,第 3 次代乳成功,获得仔鼠 18 只,存活率 88%,离乳存活率 66%,依照 GB / T 14926. 41-2001 进行无菌金黄地鼠状态检测均为合格。 结论 通过剖宫产净化技术,使用无菌级 ICR 小鼠和无菌级 SD 大鼠进行代乳,达到微生物净化目的,获得无菌化金黄地鼠模型。

    Abstract:

    Objective A sterile golden hamster model was established by cesarean section purification. Methods SPF-grade donor female golden hamsters were selected, and males and females were mated 1 ∶ 1 and separated after mating. The cage time of the surrogate mothers was 1 week earlier than that of the donor mothers. Parturient golden hamsters underwent hysterectomy on a sterilized workbench, and the uteruses were transferred into isolation kits and stripped. To obtain sterile milk for milk replacement, sterile ICR mice and sterile SD rats were used. After successful separation, the hamsters were transferred to isolation kits to prepare for feeding. The sterility status of the feeding isolation kits was tested monthly. Results Three caesarean sections were performed, but the first and second lactations failed. The third milk replacement was successful, and 18 young hamsters were obtained with survival rates of 88% and 66% after weaning. All hamsters were quality tested by GB / T 14926. 41-2001. Conclusions Using a cesarean section purification technique and sterile ICR mice and SD rats for microbial-free milk replacement, a sterile golden hamster model was obtained.

    参考文献
    相似文献
    引证文献
引用本文

郭亚茜,杜晓鹏,刘凯慧,王昭华,朱 华.无菌金黄地鼠模型建立[J].中国比较医学杂志,2024,34(8):87~93.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-04-08
  • 在线发布日期: 2024-10-15
您是第8774211 位访问者
版权所有:中国比较医学杂志 主管单位：中国科学技术协会 主办单位：中国实验动物学会 中国医学科学院医学实验动物研究所
地址：北京市朝阳区潘家园南里5号 邮 编 ：100021 电话：010-67779337 E-mail:bjb@cnilas.org
技术支持：北京勤云科技发展有限公司
防诈骗提示！请勿点击不明链接或添加个人微信。编辑部所有邮箱后缀均为@cnilas.org
关闭