1.川北医学院基础医学与法医学研究所,四川 南充 637100;2.川北医学院附属医院病理科,四川 南充 637100;3.川北医学院附属医院肝胆外科,四川 南充 637100
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1. Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong 637100, China.2. Department of Pathology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637100. 3. Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637100
目的 分析线粒体转录因子B1(transcription factor B1,mitochondrial,TFB1M)在肝癌细胞中的生物学作用,初步探讨其调控HCC恶性转移的分子机制。 方法 基于TCGA-LIHC(liver hepatocellular carcinoma)和GEO肝癌相关数据库分析TFB1M在肝癌中的表达水平及其与肝癌患者临床特征的关系;基于TFB1M表达水平中位值,将TCGA-LIHC样本分为TFB1M低表达组(n=170)和TFB1M高表达组(n=170),以高表达组为对照组,利用GSEA进行基因集富集分析;构建过表达TFB1M质粒,转染肝癌HepG2和Huh7细胞。采用划痕愈合、Transwell小室等检测过表达TFB1M对肝癌细胞迁移和侵袭的影响。 结果 与正常肝组织相比,TFB1M在肝癌组织中表达水平明显降低;且TFB1M表达水平与肝癌患者性别有关。GSEA富集分析结果显示,TFB1M低表达组主要与肝癌增殖增强基因集、肝细胞癌干性增强基因集和细胞外基质组装基因集有关。细胞生物学实验结果显示,与空载组相比,过表达TFB1M后,肝癌细胞迁移和侵袭能力均下降。 结论TFB1M在肝细胞癌中呈显著低表达,过表达TFB1M能够显著抑制肝癌细胞迁移和侵袭。
Objective To explore the biological effect of transcription factor B1, mitochondrial (TFB1M) on hepatocellular carcinoma (HCC) cells, and to explore the molecular mechanism of its regulation on malignant metastasis of HCC. Methods The expression level of TFB1M in liver cancer and its relationship with clinical characteristics of patients with HCC was analyzed based on TCGA-LIHC and GEO databases. Based on the median expression level of TFB1M, the TCGA-LIHC samples were divided into low expression group of TFB1M (n=170) and high expression group of TFB1M (n=170), and GSEA was used for gene set enrichment analysis.The overexpressed TFB1M plasmid was constructed to transfect HepG2 and Huh7 cells. Cell migration and invasion were assessed by scratch healing and Transwell chamber assays. Results The expression of TFB1M was significantly decreased in HCC, and the expression of TFB1M was related with gender in HCC. The Results of GSEA analysis indicated that the low expression of TFB1M might be related to liver cancer subclass proliferation up gene set, stem cell hepatocellular carcinoma gene set, and extracellular matrix assembly gene set in HCC. Overexpression of TFB1M remarkably attenuated the migration and invasion activity of HCC cells. Conclusions The expression of TFB1M was significantly decreased in HCC, and overexpression of TFB1M could attenuated the migration and invasion activity of HCC cells.
陶 佳,何琳莉,王 莉,戈鹏圣,蹇顺海.过表达TFB1M抑制肝癌细胞迁移和侵袭[J].中国比较医学杂志,2024,34(9):76~82.
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