miR-204-3p 干预二氧化硅粉尘诱导的大鼠矽肺上皮间质转化进程
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宁夏医科大学公共卫生学院,银川 750004

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R-33


miR-204-3p affects silica dust-induced epithelial mesenchymal transition in a rat silicosis model
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School of Public Health, Ningxia Medical University, Yinchuan 750004, China

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    摘要:

    目的 探索 miR-204-3p 在矽肺上皮间质转化( epithelial-mesenchymal transition,EMT)中的作用,阐明 miR-204-3p 通过调控二氧化硅粉尘诱导的大鼠肺泡 EMT 过程进而影响矽肺纤维的机制。 方法 取40 只 SD 大鼠随机均分为 4 组:对照(Control)、矽肺(Silicosis)、AAV-Control 和 AAV-miR-204-3p 组。 苏木精-伊红(HE)、Masson 染色进行大鼠肺组织损伤的病理学观察;实时荧光逆转录定量 PCR(RT-qPCR)法检测各组大鼠肺组织中 miR-204-3p 和 EMT 标志物基因相对表达水平;Western blot 法检测各组大鼠肺组织中 EMT相关标志物蛋白表达水平。 结果 与对照组相比,矽肺组肺泡结构损伤,肺间隔间质纤维化,间充质标志物表达升高(P<0. 05,P<0. 01,P<0. 001);与 AAV-Control 组相比,AAV-miR-204-3p 组肺泡结构较完整,EMT 过程缓解,纤维化得到改善,间充质标志物表达降低(P<0. 05,P<0. 01,P<0. 001)。 结论 游离的二氧化硅粉尘可诱导大鼠肺组织 EMT,过表达 miR-204-3p 对游离二氧化硅粉尘所致大鼠 EMT 过程具有干预作用,有望影响矽肺纤维化进程。

    Abstract:

    Objective To explore the role of miR-204-3p in silicosis and to elucidate the mechanism by which it affects silicosis fibers by regulating silica dust-induced alveolar epithelial-mesenchymal transition (EMT) in rats. Methods Forty SD rats were divided randomly into 4 groups: Control, Silicosis, AAV-Control, and AAV-miR-204-3p groups. The pathology of lung tissue damage was detected by hematoxylin and eosin ( HE) and Masson staining. Relative expression levels of miR-204-3p and EMT marker genes in lung tissues from rats in each group were analyzed by real-time fluorescence reverse transcription quantitative PCR(RT-qPCR), and protein expression levels of EMT-related markers in lung tissues were detected by Western blot. Results The alveolar structure was damaged,the lung septa showed interstitial fibrosis, and expression levels of mesenchymal markers were elevated in the Silicosis group compared with the Control group (P<0. 05, P<0. 01, P<0. 001). The alveolar structure was more complete,the EMT process was alleviated, fibrosis was improved, and mesenchymal marker expression was reduced in the AAVmiR-204-3p group compared with the AAV-Control group (P<0. 05, P<0. 01, P<0. 001). Conclusions Free silica dust induces EMT in rat lung tissue. Overexpression of miR-204-3p can attenuate the EMT process induced by free silica dust in rats, and may thus affect silicosis fibrosis.

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俞 静,陈 芳,胡文选,皮洋阳,张 玺,王露凝,赵 萍,王发选. miR-204-3p 干预二氧化硅粉尘诱导的大鼠矽肺上皮间质转化进程[J].中国比较医学杂志,2025,35(3):40~47.

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  • 收稿日期:2024-09-11
  • 在线发布日期: 2025-05-29