Traditional laboratory animal breeding is costly and time-consuming, thus limiting experimental zoology teaching practices. Zebrafish have unique biological characteristics and have become widely used as an experimental animal model; however, teaching examples related to zebrafish in experimental zoology courses are still scarce. This review explores the application of zebrafish by incorporating them into the “Tissue Sectioning Techniques” and “Immunological Techniques” modules in the “Medical Laboratory Animal Science and Technology” course. The aim is to utilize the advantages of zebrafish in experimental zoology teaching and to explore their potential applications. This study provides a detailed introduction to the experimental procedures, experimental design, and teaching content involving zebrafish, with the aim of offering undergraduate students a more efficient, cost-effective, and practically meaningful experimental teaching platform.