Anatomy of laboratory animals plays an important role in laboratory animal science, veterinary medicine, and other life sciences. Integrating mind mapping into the entire teaching process can effectively help teachers to optimize the organization of teaching contents and stimulate students’ enthusiasm and self-motivation for learning. This manuscript aims to explore the feasibility, advantages, and challenges of applying mind-mapping tools for teaching anatomy of laboratory animals. We analyze the different application scenarios from the perspectives of both teachers and students, with the aim of providing practical teaching tools and methodologies to enhance instructional effectiveness in the teaching of anatomy of laboratory animals.