大鼠物体识别实验装置的研制
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国家“重大新药创制”专项(2012ZX09J12201)、国际科技合作专项-人参益智药效与基因/蛋白表达谱关联规律合作研究(2011DFA32730)、航天医学基础与应用国家重点实验室开放课题(SMFA 10K01)。


The development of the apparatus for Object Recognition Task in rats
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    摘要:

    【摘要】 目的 研制一种可靠、稳定的用于检测大鼠识别记忆的行为学装置。方法 结合机械工程和实验动物行为学技术研制能够模拟自然环境、封闭的大鼠物体识别实验装置,并用正常大鼠进行物体识别实验对其可行性进行验证。结果 大鼠在封闭装置和传统敞口装置中对新奇物体探索时间都显著大于对熟悉物体探索时间(P<0.01, P<0.05),并且封闭装置中大鼠的相对辨别指数明显大于0(P<0.01);大鼠在封闭装置中测试期探索总时间、绝对辨别指数与传统敞口装置组比较都有显著性差异(P<0.05),而相对辨别指数无差异。 两种常用体重大鼠在新装置中对新奇物体探索时间显著大于对熟悉物体探索时间(P<0.01),相对辨别指数也都大于0(P<0.01,P<0.05),并且两组的相对辨别指数无差异。结论 大鼠在新装置中能够辨别出熟悉物体,表现出正常的记忆能力,并且探索水平优于敞口装置组。新研制的大鼠物体识别实验装置和传统敞口装置一样都能够用于检测大鼠的识别记忆能力,并能模拟自然环境、避免外界干扰。

    Abstract:

    【Abstract】 Object Developing the apparatus for Object Recognition Task(ORT) in rats to evaluate recognitive behaviour activities in the rat. Methods Combing the advantage of mechanical engineering and animal behaviour developed the closed apparatus stimulating the nature environment for ORT in rats and validated its feasibility with ORT in rats. Results Both of 230±10g rats in the closed apparatus and open-field apparatus could discriminate the familiar object from the novel one in the test phase(P<0.01, P<0.05). Moreover,the relative discrimination index was significantly different from the chance level(0)in the closed apparatus groups(P<0.01),the total exploration time for the objects in the test phase,and the absolute discrimination index of rats in the closed apparatus group were significant, if compared with open-field apparatus group(P<0.05),however, the relative discrimination index was not significant.Meanwhile,both of male Wistar rats (230±10g and 340±10g) could discriminate the familiar object from the novel object in the test phase(P<0.01).The relative discrimination index was significantly different from the chance level(0)in the two groups(P<0.01, P<0.05), but there was not significant, if compared with each other. Conclusion The normal rats in the closed apparatus could discriminate the familiar object from the novel one in the test phase,performing the memory for the familiar object ,but the exploration level was better than the open-field group.The new closed apparatus stimulating the nature environment was the same as the open-field apparatus being used for ORT in rats .

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宋广青,高莉,孙秀萍.大鼠物体识别实验装置的研制[J].中国比较医学杂志,2013,23(8).

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  • 收稿日期:2013-01-04
  • 最后修改日期:2013-03-28
  • 录用日期:2013-07-25
  • 在线发布日期: 2013-08-29
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