Objective To establish a mink model of canine distemper virus, and different canine distemper virulent strain virulence was evaluated by the mink animal model for laying the foundation for the study of the canine distemper virus. Methods Different canine distemper viruses were separated from specimens ,cell-cultured, passaged and determined virulence of CDV. And we compared the virulence of different generation virulent strains, stable virulent strains were used for establishing mink canine distemper animal model and evaluated their virulence. Results we screened 3 canine distemper virulent strains and made dog animal experiments, the three viruses infected dogs and they showed a strong clinical symptoms, and mink animal model was established using different generations. Conclusion: we successfully established the canine distemper animal model and evaluated virulence of CDV.