Objective To investigate the reproductive performance and compare pain threshold of ASIC3-/- and TRPV1-/- mice with C57BL/6 mice. Method: Twelve ASIC3-/- mice, twelve TRPV1-/- mice and twelve C57BL/6 mice were chosen and monogamously mated. There are 6 male and 6 female mice in each group, with age of 8-10 weeks. The pregnancy rate, the litter size, and the weaning rate of the ASIC3-/- mice and TRPV1-/- mice were analyzed. The pain threshold among the C57BL/6 mice, the ASIC3-/- mice and TRPV1-/- mice were compared. Result: The pregnancy rate, the litter size, and the weaning rate of the ASIC3-/- mice is slower than that of the TRPV1-/- mice. Comparing with C57BL/6 mice，the tactile threshold of ASIC3-/- mice was significantly higher (P<0.001) and the thermal threshold of TRPV1-/- mice was significantly higher (P<0.001). Conclusion The reproductive performance of the ASIC3-/- mice is weaker than that of the TRPV1-/- mice. The tactile sensitivity of the ASIC3-/- mice decreased. And the thermal sensitivity of the TRPV1-/- mice decreased.