Objective Rat is a common animal for making heart disease model. The magnetic resonance imaging (MRI) is becoming an important technology for study of pathological process and drug evaluation with animal model. However, no normal cardiac MRI technological parameters of rats are available at home and aboard, and limit this technology application. In this work, the technological parameters for structure and function of left and right ventricle from normal adult rats of Wistar, Sprague-Dawley and Lewis were acquired and analyzed quantitatively by magnetic resonance imaging (MRI), and provided as reference data for heart disease model preparation and analysis. Methods The cardiac CINE was used in a 7.0T high field MRI to assay the structure and function parameters of left and right ventricle of the rats. Results The structure and function parameters of left and right ventricle of the rats were acquired and analyzed, including left and right ventricle end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF); left ventricle papillary muscle lay end-diastolic diameter (EDD), end-systolic diameter (ESD), fractional shortening (FS), end-diastolic anterior wall thickness (EDAWT), end-diastolic posterior wall thickness (EDPWT), end-systolic anterior wall thickness (ESAWT), end-systolic posterior wall thickness (ESPWT), anterior wall thickening (AWT) and posterior wall thickening (PWT), cover right ventricle papillary muscle lay end-diastolic wall thickness (EDWT), end-systolic wall thickness (ESWT) and wall thickening (WT). Conclusion This study obtained quantity information of 18 cardiac structure and function parameters of three normal rat lines, which can be used as reference data for heart disease model preparation, pathological process and drug evaluation.