特殊染色及免疫组化双重染色在恒河猴糖尿病模型中的应用
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国家高技术研究发展计划(863课题)


The Use of Special and Double Immunohistochemistry staining in Experimental Acaques Diabetes Model
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the National High Technology Research and Development Program of China

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    摘要:

    目的 在链脲佐菌素(STZ)诱导恒河猴糖尿病动物模型基础上,用特殊染色、免疫组化双染方法显示胰腺等组织中的特征性病变。方法 健康恒河猴5只,小剂量(30mg/kg)多次静脉注射STZ,濒死状态时将动物安乐死。取胰腺、心脏、肾脏、脾脏、肝脏、眼球、脑等器官制成石蜡切片,用H.E,PAS,masson,天狼星红和甲苯胺蓝等方法进行特殊染色,用免疫组化双重染色法同时显示胰岛A、B细胞。结果 模型动物的胰岛萎缩,数量减少。Masson染色见外分泌部间质内纤维增生。局灶性慢性肾炎,病变区肾间质纤维组织增生,部分肾小球及肾小管萎缩。天狼星红染色见脾脏中央动脉管壁增厚。免疫组化双染见胰岛A胰高血糖素表达增多,胞浆呈棕褐色。B胰岛素表达减少,胞浆粉红色。结论 H.E染色结合特殊染色和免疫组化双重染色可较好地对STZ诱导糖尿病动物模型进行组织学评价。

    Abstract:

    Objective Use the special and double immunohistochemistry staining method to obsearve the typical histopathological change in experimental acaques diabetes model.Methods we used STZ (30 mg/kg of body weight, intravenously, 3days)to five macaques. The animal was sacrificed when impending death. The histopathological variety of pancreas,heart,kidney and spleen were observed with H.E,PAS,Masson,Sirius red,toluidine blue and double immunohistochemistry staining. ResultsThe quantity of pancreatic island decreaseded obviously,the reminders showed atrophy. fiber hyperplasia were observd in exocrine pancreaswith masson stain. In kidney, There were focal chronic nephritis, renal interstitial fibre hyperplasia, parts of glomerular and renal tubular atrophy.Small arteria in spleen was thickening with PAS stain. In double immunohistochemistry staining, the quantity of brown-colored A cell is increased while the pink-colored B cell is decreased. Concusion The use of H.E combined with special and double immunohistochemistry staining is a better method to evaluate the Diabetes Mellitus model.

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朱华.特殊染色及免疫组化双重染色在恒河猴糖尿病模型中的应用[J].中国比较医学杂志,2013,23(2).

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  • 收稿日期:2012-12-21
  • 最后修改日期:2013-01-07
  • 录用日期:2013-01-09
  • 在线发布日期: 2013-03-05
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