国家科技支撑项目课题（2012BA131B06）
目的 研究GDF-9和BMP-15在不同品系、不同繁殖能力小鼠中的多态性和作用机制，找出繁殖力与基因之间的关系。方法 采用直接测序法筛查多态性位点，并对多态性位点构建双荧光素酶报告基因载体,检测5’UTR对下游基因的影响。结果 ICR小鼠的产仔数是BALB/c的2.8倍左右；在ICR和BALB/c中分别发现了GDF-9和BMP-15的三个多态性位点，一个位于5’端的非编码区，另外两个位于蛋白编码区；双荧光素酶检测结果显示GDF-9的5’UTR区可以促进下游基因的表达。结论 GDF-9在ICR和BALB/c这两个品系小鼠中存在多态性位点，这些位点的变化对下游基因的表达起一定的促进作用。
Objective To study the polymorphism and mechanism of GDF-9 and BMP-15 in mice of different strains, different reproductive capacity, and find out the relationship between genes and fecundity. Methods Screening polymorphism loci by using direct sequencing method, and testing 5 'UTR impact on downstream genes by constructing luciferase dual reporter vector. Results ICR mice litter size is about 2.8 times the BALB/c; In ICR and BALB/c, respectively, found three polymorphisms, one at the 5 'end of the non-coding region, the other two located in the protein coding region ; Dual-luciferase test results show that the 5'UTR area of GDF-9 can promote the expression of the downstream gene. Conclusions GDF-9 polymorphic loci exist in ICR and BALB/c strains, changes in these sites play a certain role in promoting the expression of the downstream gene.
殷昆仑. GDF-9和BMP-15在不同品系小鼠中的多态性和生殖能力关联分析[J].中国比较医学杂志,2013,23(3).
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