Objective:Based on the environmental testing results of the 2012 annual laboratory animal license in Hubei Province, we will discuss the results and make suggestions to promote the reliable operation of the experimental animal facility in Hubei Province.Methods:The environment was monitored in 30% of the regional sampling way on the basis of GB14925-2010 Laboratory animal.Result:In addition to the air velocity and maximum average concentration Settlement bacteria, other indexes don’t meet national standards..Conclusion:To strengthen Laboratory animal facility management and training and improve the level of management overall improve the environmental conditions of laboratory animal facility in Hubei Province.