Objective The experiment investigates the influence of the Low-Intensity Pulsed ultrasound Stimulation to restore the segment bone defect. Methods Insert 12mm diametric 20mm long TiC/Ti Foam into the area of Beagle dogs’ left tibia with segmental bone defect. Divide the six dogs into two groups randomly, the experiment group with LIPUS (1.5MHz, ISATA30mW/cm2, pulsed width 200μs, repeating 1kHz pulsation, once/d), the contra group without LIPUS. Observe and analyse formation around the material with X-ray film and the admeasurement of the bone mineral density at 4w and 8w. Results Six Beagle dogs were included in the final analysis. At week 4, the maturation of newly formed bone under the LIPUS treatment group appeared earlier than that of the contra group, on the basis of the bone mineral density increase and bone callus growth. On the bone mineral density aspect, the experimental group’s rate is higher than the contra group; on the bone callus aspect, the experimental group grow from marginal to central, the contra group still has vacancy. At week 8, the skeletal structure stability under the LIPUS treatment group is higher than that of the contra group, on the basis of the bone callus area and the formability. Measured the bone mineral density at week 4, the experimental group is higher than the contra group(p<0.05). Conclusion Combining applying the Low-Intensity Pulsed Ultrasound Stimulation and the artificial bone material could enhance the formation rate of the bone callus and the bone mineral density, and shorten the healing time of the segmental bone defect.