Objective Construction of a type 2 diabetic (T2DM) model in rhesus monkey, so that to become the effective substitute for the study of human T2DM. Methods Based high glucose and fat diet, to appearance hyperlipidemia and obesity status followed by injection of 35 mg/kg of streptozotocin (STZ). The body mass index(BMI), lipid profile, fasting blood glucose, insulin, insulin resistance index, urine sugar and oral glucose tolerance test were measured, and some of its clinical features were analyzed. Results The BMI greater than 35 mg/kg reach to severe obesity shown a characteristics of hyperlipidemia, blood glucose, insulin and insulin resistance index were significantly elevated (P <0.01), urine sugar test were positive, and glucose tolerance were impaired, and fasting glucose of higher than 7 mmol/L and glucose levels higher than 11.1mmol/L in two hours in the T2DM model group. Conclusion Analysis some of the clinical features, the model group had the typical T2DM clinical features. So it will be an effective model in human T2DM research.