Oxidative stress and damage is closely related to skin functional decreased and diseases. Traditional biological evaluation of the skin antioxidant often use animal model through creative a model of local skin injury or disease. With the deepening understanding of the mechanism of skin oxidative damage and the development of in vitro culture techniques, using cultured skin cells or reconstructive skin model test system can be establish a new rapid testing method which can be used in the evaluation and screening of antioxidant effects, as well as mechanisms research and product development.