Objective To investigated the therapeutical effects of Prescription No. 1 (PN-1) on Alzheimer’s disease by KinexTM Antibody Microarray which is a kind of high-throughput antybody microarray. Methods The Alzheimer’s disease mouse models are divided into treatment group and control group. There are 8 mouse in the treatment group and given once PN-1 every day by intragastric administration for 3 months，The 4 in the control group are given double distilled water every day by intragastric administration for 3 months too.The different expression protein between the two groups are screened by KinexTM Antibody Microarray , the molecules relevant Alzheimer’s disease is determined by Ingenuity Pathway Analysis（IPA）and critical review of literatures. Results Compared to control group, the proteins of the mouse in the treatment group which are associated with Alzheimer’s disease and have different expression between the two groups : p- tau（T522）、COX2、caspase6 and caspase9 which are decreased；bcl-xl and CaMK4 which are increased. Conclusions Prescription No. 1 (PN-1) can ameliorate the pathophysiological performance of Alzheimer’s disease mouse models by inhibiting the hyperphosphorylation of tau , inflammatory response,neuronal apoptosis and improving memory, is multitargeted，and may be an effective drug treatment for Alzheimer’s disease.