Abstract : Objective To compare an optimized method of transverse aortic constriction (TAC) without ventilator and the traditional TAC by the survival rate of mice in one week. Methods Ninety C57/BL6 mice were randomly divided into sham-operated group (n=30), TAC without ventilator group (n=30) and TAC with ventilator group (n=30). The survival rates were recorded in 1 week and the cardiac function was evaluated by using ultrasonic instrument 4 weeks after the operation. Results The mice survival rate was higher in the TAC group without ventilator than in that with ventilator, and the new improved method resulted in the same cardiac hypertrophic model after 4 weeks. Conclusions The new optimized method of TAC without ventilator is better for improving survival rate and more convenient than the traditional TAC.