【Abstract】: Objective: To establish a method for the detection of mRNA transcription of SLAM as CDV receptors in different tissues of canine. Method: By using GAPDH as the house keeper, The △△Ct relative quantification method was used to detect the transcription levels of SLAM mRNAs in different tissues of canine. Result: The inter-assay variability (CV %) were0.89 %- 2.35 %. The results indicated that the qPCR had advantages of high reproducibility. Results of the study showed that the SLAM mRNA were transcripted at high level in the spleen, hilar lymph node, lymphonodi mesosteniales, inguinal glands and low level in the urinary bladder. Conclusion: The quantitative RT-PCR method was established successfully for the detection of mRNA transcription of SLAM in different tissues of canine.