Objective To construct metastasis-associated tumor gene 1(MTA1) stably-over-expressing mouse model by inserting the full human MTA1 CDS to the genome of C57BL/6J mouse. Method MTA1 gene was cloned form human with RT-PCR, The cloned MTA1 gene was then inserted eukaryotic expression vector pcDNA3.1 to construct of pcDNA3.1-MTA1 carrier. The transgenic mouse were produced by microinjection method and the genotype was detected by PCR with specific primers. The expression profiles of MTA1 in body tissues were illustrated by Western-blot and immunohistochemical . Results Three hundred mouse zygotes were treated with transgenic vectors DNA by microinjection and then transplanted into ten artificial pregnant female mice. Ten of these mice were gravid . The transplantation rate was 100% .A total of 80 F0 mice were obtained, of which 9 were carrier MTA1 gene screened by PCR. The positive rate of transgenic mice was 11.25% (9/80) . Four lines of MTA1 transgenic mouse were established. MTA1 in transgenic mouse with tissue-specific distribution and the expression levels of the MTA1 gene in brain, liver, lung and colon from transgenic mouse were higher than those form control mice.But, kidney and skeletal muscle showed no difference. Conclusion Brain, liver, lung and colon of stably-over -expressing MTA1 transgenic mouse were established successful. To further MTA1 research has laid a good research model.