[Abstract] Objective To explore the management of anesthesia of the Bama minipigs for major hepatectomy. Methods 30 healthy male Bama minipigs were chosen for hepatic trisegmentectomy. Ketamine 12mg/kg were given by intramuscular for basic anesthesia before anesthesia induction and intubation using pentobarbital sodium 24mg/kg. Isoflurane inhalation anesthesia was used for anesthesia maintenance. Anesthetics dosages were adjusted according to the change of hemodynamics. And the mechanical ventilation parameters were regulated in accordance with SPO2 and PETCO2. Results 26 of the 30 Bama minipigs get through the perioperative period successfully. The range of the mean arterial pressure (MAP) was less than 20% and the jugular venous pressure (JVP) was kept at 3-6mmH2O during the operation. And the SPO2 was kept above 95% throughout the operation. Adverse event was occurred on 3 pigs. Conclusion The management of anesthesia of the Bama minipigs for major hepatectomy needs appropriate drug and fluid management, complete monitoring, and properly handling the abnormity of physical signs. The hemodynamics stability during the hepatectomy and the treatment of the residue liver are of great importance to anesthesia management.