【Abstrct】Objective To explore the two different methods of repeated cryo-injury and coronary artery ligation to establish rats model of acute myocardial infarction(AMI)，and to compare the rats’ mortality and parameters of cardiac function.Methods: Forty SD rats (males 20) were randomized into two groups:cryo-injury group(CIP)(n=20)and coronary artery ligation group (CAL)(n=20). Cryo-injury of the rats’ left ventricle free wall was performed with the use of a round copper probe cooled by immersion in liquid nitrogen without ventilator support. It was applied to the LV free wall three times for each five seconds. The CAL group was ligated anterior descending coronary with ventilator support. We observed the rats’ mortality during operation, post-operation period and 28-day. Parameters of cardiac function were observed at the 28-day(SAP、DAP、LVEDP、LVMI、眃p/dtmax). Results ①The death number of rats in CIP group has no statistical significance in intraoperative、postoperative、28 days compared with the CAL group(P>0.05); Compared with CAL group，the rats total mortality of CIP group was obviously decreased(P<0.05).. Rats death amount of CIP group was obviously less than CAL group; ②At 28-day,in CIP and CAL groups，the cardiac function parameters (SAP、DAP、LVEDP and LVMI)were increased and 眃p/dtmax was decreased，The result showed two groups of rats cardiac function deterioration.There was no statistically significance in cardiac function (P>0.05).Conclusions: Repeated cryo-injury method deserves recommendation due to the advantages, such as safety and low mortality without ventilator support to establish rat model of acute myocardial infarction．