[Abstract] Objective To establish the drug-resistant Candida albicans disseminated infected mice model, and to provide an important tool for drug screening in the drug-resistant Candida albicans. Methods The clinical Drug-resistant Candida albicans CaR was injected via the lateral tail vein of immunosuppressive ICR mice to establish a mouse model of disseminated candidiasis resistant. The model was evaluated by the model evaluation index, such as the clinical features, survival condition, the amount of tissue fungal burden, tissue pathology, cytokines content and efficacy evaluation. Results Mice infected with drug-resistant Candida albicans CaR died at the first days after the inoculation, and mortality rate was 90.7% within 16 days. There was no significant difference between CaR group and the clinical drug sensitive strain CaS group, however, CaR group mice died faster than CaS group in the early stage; On the fourth day of infection, Candida albicans was detected in the tissues, and the difference was significant between groups CaR and CaS in kidney and brain;The pathological sections were observed, and the main manifestations were fungal granuloma in kidney, brain and heart;11 kinds of cytokines in renal tissue were detected by liquid phase chip technology;The changes of IL-1α,IL-6,IL-10,TNF-αand IFN-γin kidney were significant. Compared with CaS group, IL-1 and IFN-γ were significantly higher and TNF-αdecreased significantly in CaR group.The mice of groups CaR and CaS were treated with 10mg/kg fluconazole, the mortality rates were 37.5% and 83.3%, which have a significant difference. Conclusion Drug-resistant Candida albicans CaR were injected via the lateral tail vein of immunosuppressive ICR mice to establish the disseminated infected mice model successfully.