Objective Shkbp also called Shkbp1, can competitively inhibit binding CIN85 and c-Cbl, thereby blocking the epidermal growth factor receptor (EGFR) endocytosis and degradation, to play its role of tumor promotion. This article aims to explore Shkbp1 deletion (Shkbp-1 - / -) mice Changes in blood, bone marrow, spleen and blood cell classification T cell subsets. Methods Shkbp-1 - / - transgenic mice were identified by PCR genotype; blood tests using automatic classification of blood cells was measured using flow cytometry Shkbp-1 - / - and control mice blood, bone marrow, and spleen T lymphocyte subsets. Results Blood routine examinations showed that: neutrophils and eosinophils tended to increase, and there are significant differences. Streaming results showed that: the control group reduced blood CD4 CD8 double positive cells in the bone marrow CD3 , CD4 increased. In the spleen tissues show its CD3 , CD4 , CD8 , CD4 CD8 double positive cells were decreased. Conclusion Shkbp1 involved in the maturation and differentiation of blood cells, which affects the number of immune cells. This study lays a foundation for the study of how Shkbp1 is involved in the differentiation of blood cells.