Abstract:Objective Localization of iRhom2 and its mutant proteins with uncv mice in Vero cells by PKH26 combined with Hoechst33258.Methods The cell membrane was stained with PKH26, and the nuclei were stained with Hoechst33258 dye.Then observed under the laser scanning confocal microscope.Results It is found that wild iRhom2 are distributed in the cytoplasmic region,and its iRhom2mut both in cytoplasmic region and nucleus.Conclusion The study speculate deletion in N- terminal of iRhom2 affect its subcellular localization.