协和青年基金（编号：3332015196）和卫计委公益性行业科研专项（编号：201302006）
摘要： 目的 猴B病毒（Monkey B virus, BV），也称猴疱疹病毒I型（Cercopithecine herpesvirus 1），是重要的人兽共患病原。国家标准检测猴B病毒作为抗原，但由于生物安全问题，其抗原制备受到很大限制，因此使用替代抗原进行其血清学检测并对其比对验证。方法 应用2种ELISA方法（抗原分别为BV和HVP2）和1种IEA方法（抗原为HSV-1）对本实验室135份送检恒河猴血液样品进行筛查，对阳性及可疑样品再以IFA和Western Blot方法（抗原为HSV-1）以及以HSV-1 gC1纯化糖蛋白为抗原的免疫印迹方法验证。结果 HVP2-ELISA、BV-ELISA和HSV-1-IEA阳性检出率分别为32.6%、37.8%和34.8%；3种方法检测结果一致的样品占91.1%（123/135），阳性结果可被IFA和WB确证；可疑样品12份，33.3%（4/12）的样品经验证检验为阳性。结论 与BV抗原相比，替代抗原HSV-1的敏感性和特异性较HVP2更为接近；阳性样品及可疑样品的确证检验应使用多种方法，避免漏检。
【Abstract】 Objective Monkey B virus(BV), also known as Cercopithecine herpes virus, was significant zoonotic pathogen. Alternative antigens were usually used in the detection of B virus from rhesus monkey sera, because of its strict demand for preparation. But the accuracy of these different detection methods using alternative antigens still need to be verified. Method 135 sera samples from rhesus were detected by HVP2-ELISA 、BV-ELISA and HSV-1-IEA, those candidate indeterminate samples and positive samples were then verified by HSV-1-IFA、HSV-1-WB and HSV-1 gC1 immunoblotting．Results The B virus positive rate of rhesus monkeys were 32.6% by HVP2-ELISA, 37.8% by BV-ELISA and 34.8% by HSV-l-IEA respectively. 91.1%（125/135） of the 135 samples tested by the three methods were consistent, and the results of all samples above could be confirmed by WB and IFA. The rest indeterminate 12 samples were further detected by HSV-1-IFA、HSV-1-WB and HSV-1 gC1 immunoblotting, 33.3%（4/12）of samples were confirmed as positive. Conclusion The sensitivity and specificity of HSV-1,as alternative antigen was relatively more closer than HVP2 to BV. In addition, positive samples and suspicious samples need to be confirmed by a variety of methods in case of leak.
李晋文.不同猴B病毒抗体检测方法的比对[J].中国比较医学杂志,2017,27().
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