微卫星DNA标记对BABL/c突变卷毛小鼠遗传特性的分析
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军队实验动物专项基金面上项目(课题编号SYDW(2016)-005)


Analysis of Genetic Characteristics of BABL/c Mutant Curly Mice by Microsatellite DNA Markers
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    摘要:

    目的 利用微卫星DNA标记技术对BALB/c突变卷毛小鼠与正常BALB/c小鼠进行遗传检测,旨在分析BALB/c突变卷毛小鼠与正常小鼠之间存在的遗传差异。方法 选取38个微卫星DNA位点结合荧光PCR技术和STR扫描基因型来检测BALB/c突变卷毛小鼠、无毛小鼠与正常小鼠三个群体的遗传变异情况。结果 38个微卫星位点在BALB/c突变卷毛小鼠与正常小鼠之间有27个微卫星位点相同,有11个位点存在差异,其突变率为28.9%(11/38);BABL/c突变无毛小鼠与正常小鼠之间有30个位点完全相同,8个位点存在差异,其突变率为21.1%(8/38);而BABL/c突变卷毛小鼠与无毛小鼠间也有12个位点存在差异。结论 BALB/c突变卷毛小鼠的突变率较高,且明显高于无毛小鼠,证明了卷毛小鼠突变与无毛小鼠突变是两个完全不同的突变系,为今后研究和开发应用BALB/c突变卷毛小鼠提供可靠的理论数据。

    Abstract:

    Objective BALB/c mutant curly mice and normal BALB/c mice were genetically detected by microsatellite DNA marker method to detect microsatellite loci between BALB/c mutant curly mice and normal mice.Methods 38 microsatellite DNA loci were selected and the variation of BALB/c mutant curly mice,BALB/c mutant hairless mice and normal BALB/c mice were detected by multiplex fluorescence PCR and STR scanning genotype.Results There were 27 microsatellite loci between the 28 microsatellite loci in BALB/c mutant curly mice and normal mice, and there were 11 loci with a mutation rate of 28.9% (11/38);there were 30 sites between BABL/c mutant hairless mice and normal mice,and there were differences in the 8 loci,then mutation rate was 21.1% (8/38);there were also 12 loci between BABL/c mutant curly mice and hairless mice.Conclusion BALB / c mutant curly mice had higher mutation rates and were significantly higher than those of hairless mice,demonstrating that mutations in curly mice and hairless mice were two completely different mutations.These results provide reliable theoretical data for the future study and development of BALB / c mutant curly mice.

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引用本文

李瑞生.微卫星DNA标记对BABL/c突变卷毛小鼠遗传特性的分析[J].中国比较医学杂志,2018,28().

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历史
  • 收稿日期:2017-07-24
  • 最后修改日期:2017-08-22
  • 录用日期:2017-09-04
  • 在线发布日期: 2018-08-16
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