Objective BALB/c mutant curly mice and normal BALB/c mice were genetically detected by microsatellite DNA marker method to detect microsatellite loci between BALB/c mutant curly mice and normal mice．Methods 38 microsatellite DNA loci were selected and the variation of BALB/c mutant curly mice，BALB/c mutant hairless mice and normal BALB/c mice were detected by multiplex fluorescence PCR and STR scanning genotype．Results There were 27 microsatellite loci between the 28 microsatellite loci in BALB/c mutant curly mice and normal mice, and there were 11 loci with a mutation rate of 28.9% (11/38)；there were 30 sites between BABL/c mutant hairless mice and normal mice，and there were differences in the 8 loci，then mutation rate was 21.1% (8/38)；there were also 12 loci between BABL/c mutant curly mice and hairless mice．Conclusion BALB / c mutant curly mice had higher mutation rates and were significantly higher than those of hairless mice，demonstrating that mutations in curly mice and hairless mice were two completely different mutations．These results provide reliable theoretical data for the future study and development of BALB / c mutant curly mice.