Objective In the traditional laboratory zoology lecture environment, there is less teacher-student interaction, less student interest and less engagement in learning.In order to further improve the teaching quality of laboratory zoology, Methods this teaching and research department based on the different teaching environment of multimedia classroom and intelligent classroom, the theoretical course teaching of Medical Laboratory Zoology as the research ,object, the course lecture format, teaching mode, teaching methods and other aspects of innovation and exploration. The study used questionnaires to understand changes in student engagement in learning and preferences for smart classroom use, and NVivo qualitative analysis software to code student classroom behaviour. Results The smart teaching environment resulted in higher student interest and more frequent teacher-student interaction in the classroom. At the same time, students are significantly more engaged in learning than in traditional teaching, with higher correct rates on in-class and post-lesson exercises and a better grasp of concepts related to laboratory zoology. Conclusions In summary, the smart teaching environment can bring students a better feeling and experience, improve their interest in laboratory zoology, increase classroom learning engagement and achieve good teaching results.