Objective: To explore the anti-inflammatory effect of Qingre Jiedu（QRJD） Formula on gout mice and its effect on gut microbiota.Method: Forty 20-22 g C57BL/6 were divided into Control group（CON）, model group（MOD）, allopurinol group（Allo）, QRJD Formula group（QRJD）, and ig 10 g/ 0.1ml carboxymethyl cellulose in blank group every morning from 1 to 28 days. Hyperuricemia mouse model was prepared by potassium oxyazinic acid (500 mg/kg) + yeast extract (10 g/kg) suspension intragaically. On the 29th day, 80ul sterile carboxymethyl cellulose was injected into the left ankle of mice in the CON group under isoflurane anesthesia, and gouty arthritis model was prepared by injecting the same amount of sodium urate solution into the left ankle of mice in the other groups. At the same time, each group was treated with corresponding drugs every day. On the 35th day, samples were taken from mice who had been fasting for 6 hours without water. Blood indexes such as uric acid, creatinine and urea nitrogen were detected. Hematoxylin-eosin staining was performed on ankle joints. The cecum contents of mice were collected and the changes of gut microbiota were detected by 16S rDNA high-throughput sequencing method. Results: ① After 7 days of treatment, compared with MOD group, QRJD formula can effectively reduce the concentrations of blood uric acid (P < 0.001), creatinine (P < 0.001) and urea nitrogen (P < 0.05), and effectively protect renal function; ② The pathological results indicated that compared with the MOD group, HE staining showed that the synovial hyperplasia and inflammatory cell infiltration were reduced in the QRJD formula group after treatment. It was found that the cartilage arrangement of the compound was more orderly than before, the cartilage destruction was less than that of the MOD group, and no matrix loss was observed.③ The immunohistochemical results of ankle joint indicated that IL-10 and TGF-β1 were not significantly increased in CON group and MOD group; Compared with MOD group, the expressions of IL-10 and TGF-β1 of QRJD recipe were increased (P < 0.05).④ In terms of biodiversity, there was no significant difference in α diversity among the four groups (P < 0.05), while β-diversity was found to be more similar to the CON group (P=0.001).⑤Compared to the CON group, the MOD group exhibited increased abundances (P < 0.05) of Ruminococcaceae spp., Dubosiella sp., Tyzzerella sp., Ileibacterium sp.and Bacteroidales spp.In contrast to the MOD group, the QRJD group showed elevated abundances (P < 0.05) of Lactobacillus sp., Ligilactobacillus sp., and Bacteroides sp. Furthermore, the interaction network of the gut microbiota indicated mutual interactions among these microorganisms.⑥In the correlation analysis between gut microbiota and renal function, as well as anti-inflammatory factors, it was observed that the relative abundance of Dubosiella sp., Tyzzerella sp.and Bacteroidales spp. was significantly positively correlated with SUA and SCR (P < 0.05). On the other hand, Lactobacillus sp., Ligilactobacillus sp., and Mitochondria spp. exhibited a positive correlation with anti-inflammatory factors IL-10 and TGF-β1, with a more significant association observed with TGF-β1 (P < 0.05).⑦COG function prediction suggested that the functions of QRJD formula group were concentrated on inorganic ion transport and metabolism, carbohydrate transport and metabolism, etc. Conclusions: QRJD effectively modulates immune inflammation and gut microbiota dysbiosis, thereby treating gout. It is hypothesized that its mechanism of gout prevention and treatment involves the regulation of gut microbiota diversity and abundance, as well as the control of the abundance of differential bacterial species such as Ruminococcaceae spp., Dubosiella sp.and Lactobacillus sp. to achieve the goal of gout therapy.