Skin modeling of transdermal drug delivery systems refers to the experimental models which can mimic the structure and function of human skin to explore and evaluate the absorption, penetration and efficacy of medicines in transdermal drug delivery. It provides alternative to traditional human skin experiments and reducing the utility of human skin in medicines research, which is convenient, controllable, and cost-effective . For the skin models of transdermal drug delivery systems, this paper introduces commonly used animal skin models, artificial skin models, and recombinant human skin models from the perspective of the transdermal absorption pathway of medicines, and analyzes its advantages and disadvantages as well as its applications, so as to provide references for the research and development of transdermal formulations and topical therapies and so on.