Objective: To investigate the modeling process and mold formation rate of N-methyl-N-nitrosourea (MNU)-induced lower jiao dampness-heat syndrome uremia bladder cancer (BC) model. Methods: Batch samples were collected by bladder perfusion (MNU) and H&E staining and pathological observation of pathological tissues were carried out at a total of 6 time points before, during and after molding, so as to understand the formation and development of carcinoma in situ of the bladder. Results: The experimental process was smooth, and no obvious urinary tract bleeding in rats occurred during the operation. By this modeling technique, considerable bladder tumors can be obtained after the eighth week, and there are obvious epithelial hyperplasia, damage and large-scale tumor formation in the bladder of rats in the model group. Conclusion: MNU can induce SD rat bladder cancer model with obvious model formation and low mortality, and the experimental data can provide a certain reference for the establishment, improvement and application of bladder cancer model.