Objective By surgery inducing Varicocele(VC) in SD rats and Apomorphine Test(APO Test),we screened the rats with Erectile dysfunction(ED) after VC,and explored the method of establishing VC and ED model.Methods Randomly divide 60 rats into control group,sham group,and model group,with 20 rats in each group.Referring to the Turner method,partially ligate the left renal vein to induce left VC.And conduct three APO Tests to screen rats with ED after inducing VC,observe and record the number of erection,genital grooming,and yawning.Measuring the diameter of spermatic vein.Weighing both testises and kidneys.HE staining and observing the pathological changes in the penis and left testis.The success rate of modeling was calculated in the model group.Results 15 out of 20 rats in the model group were successfully established VC and ED model,with a success rate of 75%.After modeling,the diameter of the left spermatic vein in the model group increased significantly and were significantly higher than before-modeling,and the diameter of the right spermatic vein in the model group increased and were higher than before-modeling.The weight of the left testises in the model group significantly decreased compared to the right testises.There was no significant difference between or within groups in the comparison of bilateral kidney weight. In the model group,the number of erections,yawning and genital grooming decreased significantly,and decreased with the time of modeling.The pathological changes of the left testis and penis were significant in the model group.Conclusions Turner method can induce the increase of the diameter of spermatic vein in rats,causing testis injury and weight loss,and APO Test can screen the rats with ED after VC induction.The combination of the two methods is suitable for establishing an animal model with VC and ED status similar to human.