Objective To evaluate the effectiveness of 1470 nm semiconductor laser therapeutic instrument (referred to as curestar therapeutic instrument) for prostatectomy in Beagle dogs. Method 28 adult male Beagle dogs were randomly divided into 3 groups: sham group (n=3), experimental group (n=15), and control group (n=10). The experimental group was further divided into 3 subgroups: 120 W/50 W, 150 W/50 W, and 160 W/50 W for vaporization cutting/coagulation hemostasis, while the control group was divided into 2 subgroups: 120 W/50 W and 150 W/50 W. 5 in each subgroup. Both the experimental group and the control group underwent canine prostatectomy through the entrance of the bladder neck under electrocision. The operational suitability and effectiveness of the product during surgery were observed. After the operation, the general condition of the dogs was observed, and blood biochemical and hematological indicators were measured at before, immediately, 3 days, 7 days, and 28 days after operation. At 1h and 4 weeks after surgery, B-ultrasound and electric resection were performed under anesthesia to observe the condition of the urethra and prostate, and the prostatic tissue was subjected to H&E staining for pathological observation. The thickness of the coagulation layer at 1h after the operation and the repair of the urethelial epithelium at 4 weeks were analyzed. Results During the operation, the experimental and control groups had good operability and showed good vaporization cutting and coagulation hemostasis performance. After the operation, there were no significant effects on the general condition, blood biochemical, and hematological indicators of the dogs. Ultrasound showed that the urethral expansion was visible immediately after the operation, and the echo of the urethral epithelium was slightly enhanced. At 4 weeks, the prostate tissue was slightly low-echo with uniformly distributed small point-like echoes inside, and the capsule was linearly high-echo, consistent with the sham group. The measurement and analysis showed that the weight of the vaporized prostate tissue in the experimental and control groups was about 0.91-1.33 g, with a resection rate of 17.11-20.27 %. And as the power of vaporization cutting increases, the laser emission time gradually decreases, while the vaporization cutting speed and efficiency both increase.However, there was no significant difference between the experimental and control groups (P>0.05). Under the electrocision microscope, a burn-like change was observed in the surgical wounds of the prostate urethra in the experimental group and the control group at 1 h after surgery, and the boundary between the wound and the normal urothelium was clearly visible. At 4 weeks, the urothelium of the prostate had been repaired and flattened, and the boundary with the surrounding normal urothelium was blurred. Similarly, pathological observations also showed that at 1 h after surgery, both the experimental and control groups showed significant damage to the prostate urethral orifice, with a small amount of carbonization and coagulative necrosis on the surface of the wound, a small amount of inflammatory cell infiltration, and a coagulation layer thickness of about 0.4 mm. At 4 weeks, the prostate urethral morphology of the sham group was normal, while the experimental group and the control group both showed new epithelial growth covering the wound, with uniform thickness and no coagulative necrosis tissue attached to the wound. Mild inflammatory reaction was still present in the surrounding area, fibroblast proliferation was obvious, and stromal and epithelial cell proliferation was visible in the surrounding prostate, some of which showed squamous metaplasia. The prostate capsule was intact and the morphology of the surrounding nerves and blood vessels was normal. Conclusion The curestar therapy instrument is effective for prostatectomy in Beagle dogs, with good vaporization cutting and coagulation hemostasis performance, and there was no significant difference in postoperative physiological indicators compared to the sham group.