【Abstract】Objective The Neuroblastoma (NB) liver transplantation model was established and compared with the adrenal orthotopic transplantation model to explore the characteristics of the model.. Methods 5×105 (SK-N-SH) cells was implanted into the left lobe of mouse liver along the long axis of liver lobe with a micro-injection needle. The growth, metastasis, expression of related genes and histopathological changes of tumor were detected after the modeling. Results The tumor formation rate of mice inoculated with tumor cells reached 100% after 21 days, and the tumor growth, metastasis, related gene expression and pathological characteristics were obvious. Conclusion In this paper, neuroblastoma liver transplantation model was successfully constructed by a relatively simple surgical modeling method, which provides more ideal model choices for future scientific experiments of NB researchers.