Japan is an important trading partner of China's import and export of experimental animals, and its quarantine supervision policy of experimental animals is meticulous and strict. In this paper, experimental dogs, cats and monkeys are taken as examples to make an in-depth analysis of the regulatory policies of major experimental animals for export to Japan. Through the current experimental animal related laws and regulations, inbound and outbound management measures, experimental animal breeding production and management situation and import and export business trend thinking, from the perfect experimental animal laws and regulations and inbound and outbound management measures, safeguard security, improve the management level of experimental animal breeding and promote experimental animal import and export trade. In order to provide reference for comprehensively improving the level of production, use and management of laboratory animals in China and strengthening the work exchange of laboratory animals between China and Japan.