Objective To explore the hearing and cochlear himorphological changes in APP/PS1 transgenic mice during the process of AD, and to determine whether the occurrence and development of AD will affect their hearing function. Methods APP/PS1 transgenic mice and littermates wild type mice were selected at the age of 4, 8 and 12 months. The changes of auditory function in APP/PS1 transgenic mice at different months were detected by immunofluorescence staining and auditory brainstem response (ABR). Results Compared with the control group, the number of Aβ plaques in the hippocampus of 4-month-old APP/PS1 transgenic mice was significantly increased, indicating that AD symptoms were already present at this time. At the age of 4, 8 and 12 months, there was no significant difference in the hearing threshold between APP/PS1 transgenic mice and wild type mice. The histomorphological examination of the cochlea showed that there was no significant difference in the key cells of the inner ear such as cochlear hair cells and spiral ganglion between the two groups of mice. ABR test results of APP/PS1 transgenic mice and littermate wild-type mice showed that the hearing threshold of both mice increased significantly with age, both of them showed age-related hearing loss. Conclusion There is age-related hearing loss in APP/PS1 transgenic mice, but the occurrence and development of AD has little effect on the hearing function of APP/PS1 transgenic mice.