CAST/EiJ小鼠外周血和脾脏中免疫细胞及亚群分析
作者:
作者单位:

1.中国医学科学院实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室;2.中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,新发再发传染病动物模型研究北京市重点实验室

基金项目:

国家自然科学基金猴痘专项,82241068;北京市自然科学基金,Z220018;中国医学科学院医学与健康科技创新工程,2021-I2M-1-037。


Immune cell composition analysis of CAST/EiJ mice
Author:
  • LIUQI 1

    LIUQI

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • HOU Yongzhi 1

    HOU Yongzhi

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • LI Na 2

    LI Na

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • ZHANG Jingjing 2

    ZHANG Jingjing

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • LU Jiahan 2

    LU Jiahan

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • CONG Zhe 2

    CONG Zhe

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • ZHU Lin 2

    ZHU Lin

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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  • XUE Jing 2

    XUE Jing

    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases
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Affiliation:

1.Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases;2.Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases

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    摘要:

    目的 探索CAST/EiJ小鼠对多种病原体易感的可能原因,分析CAST/EiJ小鼠外周血和脾脏内的免疫细胞表型,明确CAST/EiJ小鼠的免疫细胞构成。方法 利用流式细胞术,通过细胞表面标记物CD3、CD4、CD8、CD11b、CD11c、CD19、CD27、CD49b和TCRβ检测CAST/EiJ小鼠和C57BL/6J小鼠外周血和脾脏内经典型树突状细胞(cDCs)、自然杀伤细胞(NK)、B淋巴细胞、T淋巴细胞及其亚群的细胞比例。结果 CAST/EiJ小鼠与C57BL/6J小鼠的cDCs细胞比例无明显差异,但cDC1细胞亚群比例偏低;CAST/EiJ小鼠的NK细胞(主要是成熟NK细胞亚群)和T淋巴细胞(主要是CD8+T细胞)比例都显著低于C57BL/6J小鼠,而CAST/EiJ小鼠的B淋巴细胞比例高于C57BL/6J小鼠。结论CAST/EiJ小鼠中NK和T淋巴细胞比例显著低于C57BL/6J小鼠。

    Abstract:

    Objective To explore the possible causes of susceptibility to multiple pathogens in CAST/EiJ mice, immune cell phenotypes in the peripheral blood and spleen of CAST/EiJ mice were analyzed to clarify the immune cell composition of CAST/EiJ mice. Methods Classical dendritic cells (cDCs), natural killer (NK) cells, B lymphocytes, T lymphocytes and their subsets in peripheral blood and spleen of CAST/EiJ mice and C57BL/6J mice were detected by flow cytometry using the cell surface markers CD3, CD4, CD8, CD11b, CD11c, CD19, CD27, CD49b and TCRβ. Results There was no significant difference in the proportion of cDCs between CAST/EiJ and C57BL/6J mice, but the cDC1 cell subset was low. The proportion of NK cells (mainly mature NK cell subsets) and T lymphocytes (mainly CD8+ T cells) were both lower in CAST/EiJ mice than in C57BL/6J mice, while the proportion of B cells in CAST/EiJ mice were higher than those in C57BL/6J mice. Conclusion The proportion of NK and T lymphocytes in CAST/EiJ mice was lower than those in C57BL/6J mice.

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  • 收稿日期:2024-01-05
  • 最后修改日期:2024-03-09
  • 录用日期:2024-03-21
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