Objective To examine the hypoglycemic effect of Chinese yam polysaccharide on 3T3-L1 insulin resistance cell model. Methods After the creation of the IR-3T3-L1 cell model stimulated by DEX (1 μmol/L). Followed the Chinese yam polysaccharide treatment, the glucose intake and the levels of TC, TG, LDL-C, HDL-C, GSH-Px, MDA, HK and PK were measured. Additionally, the AMPK-PI3K/Akt signaling pathway"s associated genes were identified using qRT-PCR. Results The glucose consumption of IR-3T3-L1 cells was considerably decreased after 48 hours of treatment with 1 μmol/L DEX compared to the control group (P < 0.01), and the reduction persisted for 60 hours. When CYPS at 0.05, 0.15, and 0.45 mg/mL was applied to IR-3T3-L1 cells, the following outcomes were observed: the significantly increased glucose consumption (P < 0.01), HK, PK, GSH-Px enzyme activities (P < 0.01), and HDL-C content (P < 0.01). There was a reduction in the MDA activity (P < 0.01) and the concentrations of TG, LDL-C, and T-CHO (P < 0.01). Additionally, CYPS administration dramatically decreased the levels of PI3K, Akt, GLUT-4, AMPK, IRS-2, PPARa, and Adiponectin mRNA expression (P < 0.05), and reduced the IRS-1, GSK-3β, ACC, and FAS mRNA expression levels (P < 0.01). Conclusions In IR-3T3-L1 cells, CYPS can reduce oxidative stress, control lipid metabolism disorders, and enhance DEX-induced glucose intake. The AMPK-PI3K/Akt signaling pathway"s associated genes may be connected to the process.