1.中国农业科学院农产品加工研究所/农业农村部农产品加工质量安全风险评估实验室/农业农村部农产品质量安全收贮运管控重点实验室;2.西南医科大学附属中医医院中葡中医药国际合作中心;3.葡萄牙米尼奥大学生物系中葡药食植物资源研究中心
高端外国专家引进计划(G2022051012L),国家重点研发计划(2021YFD1600100)
LIU Xinyue
nstitute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety,Key Laboratory of Agricultural Products Quality and Safety Collection,Storage and Transportation Control,Ministry of Agriculture and Rural AffairsWANG Fengzhong
nstitute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety,Key Laboratory of Agricultural Products Quality and Safety Collection,Storage and Transportation Control,Ministry of Agriculture and Rural AffairsFAN Bei
nstitute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety,Key Laboratory of Agricultural Products Quality and Safety Collection,Storage and Transportation Control,Ministry of Agriculture and Rural AffairsWang Qiong
nstitute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety,Key Laboratory of Agricultural Products Quality and Safety Collection,Storage and Transportation Control,Ministry of Agriculture and Rural Affairs1.nstitute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety,Key Laboratory of Agricultural Products Quality and Safety Collection,Storage and Transportation Control,Ministry of Agriculture and Rural Affairs;2.Sino-Portugal TCM International Cooperation Center, the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University;3.Sino-PT Research Center for Medicinal and Food Plant Resources, Department of Biology, University of Minho
目的 探究山药多糖(Chinese Yam polysaccharide, CYPS)在地塞米松(Dexamethasone,DEX)诱导的3T3-L1胰岛素抵抗(IR-3T3-L1)细胞模型中的降血糖作用。方法 将IR-3T3-L1脂肪细胞随机分为对照组(Con)、模型组(DEX)、二甲双胍组(Met,阳性药组)、CYPS(0.05、0.15、0.45 mg/mL)组,通过DEX(1 μmol/L)诱导建立IR-3T3-L1细胞模型,进行给药后细胞对葡萄糖的消耗量和细胞中血脂(总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C))与丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)、己糖激酶(HK)、丙酮酸激酶(PK)含量的检测,并采用qPCR对AMPK-PI3K/Akt信号通路中相关基因表达进行检测。 结果 DEX组与Con组相比,1 μmol/L DEX处理48小时显著降低了IR-3T3-L1细胞的葡萄糖消耗量(P＜0.01),且在60 h内维持稳定。0.05、0.15、0.45 mg/mL CYPS处理IR-3T3-L1细胞显著增加了细胞中葡萄糖的消耗(P＜0.01)、HK、PK、GSH-Px酶活性(P＜0.01)、HDL-C含量(P＜0.01)；降低了MDA酶活性(P＜0.01)、T-CHO、TG、LDL-C含量(P＜0.01)。同时,CYPS可以显著回调PI3K、Akt、GLUT-4、AMPK、IRS-2、PPARa、Adiponectin的mRNA水平(P＜0.05),降低IRS-1、GSK-3β、ACC、FAS的mRNA表达水平(P＜0.01)。 结论 CYPS能够改善DEX诱导的IR-3T3-L1细胞对葡萄糖的消耗,调节脂代谢紊乱,缓解氧化应激,其作用机制可能通过调控IR-3T3-L1脂肪细胞的AMPK-PI3K/Akt信号通路来实现糖脂代谢紊乱和胰岛素抵抗调节来实现。
Objective To examine the hypoglycemic effect of Chinese yam polysaccharide on 3T3-L1 insulin resistance cell model. Methods After the creation of the IR-3T3-L1 cell model stimulated by DEX (1 μmol/L). Followed the Chinese yam polysaccharide treatment, the glucose intake and the levels of TC, TG, LDL-C, HDL-C, GSH-Px, MDA, HK and PK were measured. Additionally, the AMPK-PI3K/Akt signaling pathway"s associated genes were identified using qRT-PCR. Results The glucose consumption of IR-3T3-L1 cells was considerably decreased after 48 hours of treatment with 1 μmol/L DEX compared to the control group (P < 0.01), and the reduction persisted for 60 hours. When CYPS at 0.05, 0.15, and 0.45 mg/mL was applied to IR-3T3-L1 cells, the following outcomes were observed: the significantly increased glucose consumption (P < 0.01), HK, PK, GSH-Px enzyme activities (P < 0.01), and HDL-C content (P < 0.01). There was a reduction in the MDA activity (P < 0.01) and the concentrations of TG, LDL-C, and T-CHO (P < 0.01). Additionally, CYPS administration dramatically decreased the levels of PI3K, Akt, GLUT-4, AMPK, IRS-2, PPARa, and Adiponectin mRNA expression (P < 0.05), and reduced the IRS-1, GSK-3β, ACC, and FAS mRNA expression levels (P < 0.01). Conclusions In IR-3T3-L1 cells, CYPS can reduce oxidative stress, control lipid metabolism disorders, and enhance DEX-induced glucose intake. The AMPK-PI3K/Akt signaling pathway"s associated genes may be connected to the process.
