Objective To investigate the effect of miR-22-3p on pyroptosis of vascular smooth muscle cells induced by homocysteine. Method Human vascular smooth muscle cells (VSMC) were cultured in vitro and divided into the Control group (0 μmol/L Hcy) and the Hcy group (100 μmol/L Hcy). After intervention, Western Blot was performed to detect the expression of Pro Caspase-1, GSDMD, N-GSDMD and NLRP3. The qRT-PCR was used to measure the expression of miR-22-3p. ELISA was utilized to survey the concentration of IL-1β and IL-18 in the supernatant. After transfection with control of miR-22-3p (miR-22-3p-NC) ，mimic of miR-22-3p (miR-22-3p-mimic) , and inhibition of miR-22-3p (miR-22-3p-inhibitor) ， to observe the change of VSMC pyroptosis induced by homocysteine. Result Compared with Control group, the expression levels of Pro Caspase-1, GSDMD, N-GSDMD, NLRP3 in VSMC of Hcy Group were increased (P<0.05) , the concentration of Il-1β,IL-18 were higher (P<0.01) , and the relative expression level of miR-22-3p was lower (P<0.01) . After transfection with miR-22-3p-mimic, the expression levels of Pro Caspase-1, GSDMD, N-GSDMD and NLRP3 in VSMC decreased significantly (P<0.01) , and the concentrations of il-1β and IL-18 decreased significantly (P<0.01) . After transfection with miR-22-3p-inhibitor, the expression levels of Pro Caspase-1, GSDMD, N-GSDMD and NLRP3 in VSMC increased significantly (P<0.01) , and the concentrations of il-1β and IL-18 were higher (P<0.05) . Conclusion miR-22-3p delayed Hcy induced VSMC pyroptosis.