Diarrhea is a common and frequently occurring disease in clinic, and there are many factors causing diarrhea. At present, there are many methods to study the animal model of diarrhea, but there are limited kinds of drugs to treat diarrhea in clinic, some of the drugs available also apply to single-factor diarrhea. Therefore, the establishment and selection of diarrhea animal model is not only an important basis for further study of the pathogenesis of diarrhea, but also an effective means for clinical screening and evaluation of all-round anti-diarrhea drugs. This article reviews the establishment and evaluation of animal models of diarrhea and the progress in the research of therapeutic drugs, in order to provide reference for the animal experimental research and drug prevention and treatment of diarrhea.