Objective By optimizing the technical parameters of golden hamster embryo transfer, explore the optimal number and developmental period of transplanted embryos for golden hamster true pregnancy recipients. Methods By establishing a population of true pregnant albino golden hamsters as embryo transfer recipients, the effects of different embryonic development stages, recipient embryo reduction, and secondary transfer recipients on litter yield, donor embryo yield, and offspring survival rate were compared. Results Compared to wild-type golden hamsters as transplant recipients, true pregnancy albino receptors can quickly determine the origin of offspring and are suitable for various reproductive experimental programs based on embryo transfer. Fertilized eggs or two cell embryos have no significant impact on the transfer effect (P>0.05); The rate of donor embryos in the recipient embryo reduction group was significantly increased (22%, P<0.05), and the lower total number of births resulted in a significant decrease in cage survival rate (56%, P<0.01). The second transfer recipient"s non pregnancy rate was significantly increased (42%, P<0.01); The highest embryo yield rate (27%, P<0.01) and normal survival rate (89%) were observed in the transfer of 6-10 embryos. Conclusion The transfer of 6-10 donor embryos can help improve the yield and survival rate of donor embryos, We have successfully established an embryo transfer method using albino pregnant golden hamsters as recipients, providing technical support for the application and development of gene modification models in golden hamsters.