河南中医药大学
河南省重大专项(221100310400)； 河南省国际合作重点项目(231111521200)；岐黄学者(国中医药人教函2022-6)。
Henan University of Chinese Medicine
目的:基于中西医临床病症特点对现有的肝性脑病动物模型进行分类、分析,以期为改进和完善现有的肝性脑病动物模型提供思路,以便制备更符合临床特点中西医病症结合肝性脑病动物模型。方法:在中国知网、万方数据库,以“肝性脑病”、“肝昏迷”、“动物”、“动物模型”为关键词,在Pubmed数据库,以“hepatic encephalopathy”、“hepatic coma”、“animal model”、“animal ”为关键词进行检索,整理有具体动物模型制备方法的有效文献,根据不同造模因素和方式进行整理、分类,概括造模方法、特点,依据肝性脑病中西医临床病症特点对动物模型的临床吻合情况进行赋值、分析。结果:有效文献128篇,共11种动物模型,包含15种不同的造模方法,其中,CCl4油溶液模型吻合情况较高,西医吻合度最高的是CCl4油溶液腹腔注射+灌胃+氯化铵腹腔注射模型(吻合度92%),中医吻合度最高的是CCL4油溶液腹腔注射+乙醇水溶液自饮模型(吻合度65%),但缺乏中西医吻合度均同时较高的肝性脑病动物模型。结论:现有的肝性脑病动物模型原理均符合疾病特点,但普遍缺乏中医证候表现,缺乏中西医病症特点结合的动物模型,将中西医临床特点结合制备动物模型,有望为肝性脑病的临床诊治提供模型依据,为中西医结合治疗指明方向。
Objective To classify and analyze the existing animal models of hepatic encephalopathy based on the characteristics of Chinese and Western medicine clinical conditions, with a view to providing ideas for improving and perfecting the existing animal models of hepatic encephalopathy, so as to prepare animal models of hepatic encephalopathy that better meet the characteristics of the clinical conditions of Chinese and Western medicine combined with the conditions of hepatic encephalopathy. Methods In China Knowledge Network and Wanfang database, the Keywords "hepatic encephalopathy", "hepatic coma", "animal", "animal model" were used as Keywords, and in Pubmed database, "hepatic encephalopathy", "hepatic coma", " animal model", "animal" as the Keywords to search, collate effective literature with specific animal model preparation methods, collate and classify according to different modeling factors and methods, summarize the modeling methods and characteristics, and assess the clinical match of the animal model based on the characteristics of clinical diseases of hepatic encephalopathy in Chinese and western medicine. The clinical match of the animal model was assigned and analyzed according to the clinical symptoms of hepatic encephalopathy in Chinese and western medicine. Results There were 128 valid literature articles with 11 animal models, including 15 different modeling methods, among which, the CCl4 oil solution model had a high degree of agreement, and the highest degree of agreement in Western medicine was the CCl4 oil solution intraperitoneal injection+gavage+ammonium chloride intraperitoneal injection model (92%), and the highest degree of agreement in traditional Chinese medicine was the CCl4 oil solution intraperitoneal injection+ethanol aqueous solution self-drinking model (65%), but there was a lack of Chinese and Western medicine clinical disease characteristics.Conclusion The existing animal models of hepatic encephalopathy are all in accordance with the characteristics of the disease, but generally lack the manifestation of Chinese medicine, and lack of animal models combining the characteristics of Chinese and Western medicine. Combining the clinical characteristics of Chinese and Western medicine in the preparation of animal models is expected to provide a model basis for clinical diagnosis and treatment of hepatic encephalopathy, and point out the direction for the combination of Chinese and Western medicine in the treatment of hepatic encephalopathy.
