1.延边大学附属医院（延边医院）;2.延边大学医学院
1.Yanbian University Hospital;2.Yanbian University Medical College
目的 探讨草苁蓉纳拉苷(Boschniakia rossica boschnaloside,BRBN)对SMMC-7721肝癌细胞上皮-间质转化(epithelial-mesenchymal transition,EMT)以及侵袭、迁移的抑制作用。方法 用转化生长因子β1(transforming growth factor β1,TGFβ1)诱导SMMC-7721肝癌细胞建立EMT模型。将其分为对照组、模型组、BRBN组,利用细胞划痕愈合试验检测肝癌细胞迁移能力,Transwell侵袭试验检测肝癌细胞侵袭能力, Western blot法检测E-钙黏蛋白、N-钙黏蛋白、波形蛋白、Slug、Twist1、ZEB1及信号转导和转录激活因子3(signal transducers and activators of transcription 3,STAT3)的蛋白表达。结果 TGFβ1使SMMC-7721肝癌细胞发生纤维状间质样改变,E-钙黏蛋白表达下调,N-钙黏蛋白和波形蛋白表达上调,提示SMMC-7721细胞获得间质表型。与模型组比较,BRBN组SMMC-7721细胞侵袭率和迁移率均显著降低(P<0.05),E-钙黏蛋白表达显著升高(P<0.05),N-钙黏蛋白和波形蛋白表达显著降低(P<0.05),Slug、Twist1、ZEB1蛋白表达均显著下调(P<0.05),STAT3磷酸化水平显著降低。结论 BRBN可抑制SMMC-7721肝癌细胞侵袭、迁移,其作用机制可能与STAT3介导的EMT逆转有关。
Objective To investigate the inhibitory effect of Boschniakia rossica boschnaloside (BRBN) on epithelial-mesenchymal transition (EMT), invasion and migration of human hepatoma SMMC-7721 cells. Methods Transforming growth factor β1 (TGFβ1) was used to induce EMT model of SMMC-7721 cells. The cells were divided into the control group, model group and BRBN group. The cell migration was detected with wound healing test, the cell invasion was observed by transwell chamber assay, and the expressions of E-cadherin, N-cadherin, vimentin, Slug, Twist1, ZEB1 and signal transducers and activators of transcription 3 (STAT3) were determined with the western blot method. Results TGFβ1 resulted in the spindle-shaped changes in SMMC-7721 cells, down-regulated the expression of E-cadherin, and up-regulated the expression of N-cadherin and vimentin, suggesting that SMMC-7721 cells have obtained the mesenchymal phenotype. Compared with the model group, the expression of E-cadherin was significantly increased, expressions of N-cadherin and vimentin were reduced, expressions of Slug, Twist1, ZEB1 as well as STAT3 phosphorylation were also down-regulated in SMMC-7721 cells of BRBN group. Conclusions BRBN could inhibit the invasion and migration of human hepatoma SMMC-7721 cells, possibly by reversing EMT through STAT3 pathway.
