Objective Establishment of a three dimensional time-of-flight magnetic resonance angiography (3D-TOF-MRA) technique for observing cerebral arteries in live Mongolian gerbils. Method 1. Employ a 7.0T MRI scanner to image the cerebral arteries of Mongolian gerbils, with data subsequently processed using the RadiAnt DICOM Viewer software；2. Validate and compare imaging results via the latex perfusion method；Attempt to perform 3D reconstruction of the posterior communicating branch and surrounding cerebral blood vessels of the Mongolian gerbil and rats through analysis software; 4. Attempt to use this method to screen individuals with abnormal vascular development in Mongolian gerbils to verify the effectiveness of this method. Result 1.The 3D-TOF-MRA technology can effectively observe the cerebral arteries of living Mongolian gerbils; 2.The 3D-TOF-MRA technology has high accuracy in observing the main cerebral arteries of Mongolian gerbils, but its observation effect on finer vascular branches is not as good as latex perfusion method; 3.The data obtained from 3D-TOF-MRA technology can be used for three-dimensional reconstruction of blood vessels; 4. The application of this technology has a good effect on screening for abnormal development of arterial structure in Mongolian gerbils. Conclusion The 3D-TOF-MRA technology can be applied to the structural observation and related research of the cerebral arteries in live Mongolian gerbils.