1.苏州大学苏州医学院实验动物中心;2.黑龙江省农产品和兽药饲料技术鉴定站
1.Laboratory animal center of Suzhou Medical College,Soochow University;2.Heilongjiang Agricultural and Rural Hall
近年来，随着分子生物学技术的飞速发展，基因编辑作为能够进行基因组修饰的手段，因具有高效性、精确性和灵活性而被用于实验动物模型的建立。本文主要概述了最新的基因编辑技术在实验动物猪、非人灵长类动物以及实验动物兔、实验动物犬等动物模型的构建和应用情况，为基因编辑技术在实验大动物的广泛应用和深入研究提供理论参考，从而更好地模拟人类疾病，研究生物医学及人类复杂疾病的潜在发病机制。
Recently , with the rapidly developing of molecular biological technique, gene-editing, as a means of genome modification, has been used in the establishment of experimental animal models due to its high efficiency, accuracy and flexibility. This article mainly summarizes the construction and application of the latest gene-editing technique in animal models such as experimental animal pigs, non-human primate, and experimental animal dogs, it provides a theoretical reference for the applied widely and in-depth study of gene-editing technique in experimental large animals, so as to better simulating human diseases and study the potential pathogenesis of biomedical and human complex diseases.
