Recently , with the rapidly developing of molecular biological technique, gene-editing, as a means of genome modification, has been used in the establishment of experimental animal models due to its high efficiency, accuracy and flexibility. This article mainly summarizes the construction and application of the latest gene-editing technique in animal models such as experimental animal pigs, non-human primate, and experimental animal dogs, it provides a theoretical reference for the applied widely and in-depth study of gene-editing technique in experimental large animals, so as to better simulating human diseases and study the potential pathogenesis of biomedical and human complex diseases.