Abstract:Adipose derived stem cells(ASCs) become one of the hot seed cells in tissue engineering because of their unique advantages. An ideal method of cryopreservation of ASCs is the goal that researchers have been working on. So far, a lot of researches on cryopreservation protocols of ASCs have been done. In this review, the basical cryobiology and cryopreservation protocols of ASCs were summarized. We also looked forward to the future research interest of cryopreservation protocols of ASCs.