质子泵抑制剂泮托拉唑促进幽门螺旋杆菌SS1在小鼠胃内的定植
作者:
作者单位:

1.中国医学科学院医学实验动物研究所,中国医学科学院&2.北京协和医学院人类疾病比较医学国家重点实验室,国家人类疾病动物模型资源中心,国家动物模型技术创新中心

基金项目:

中国医学科学院中央级公益性科研院所基本科研业务费专项(2021-RC310-008;2023-PT180-01);北京市科技新星项目(Z211100002121034);国家自然科学基金青年项目(82302985)


Proton Pump Inhibitor Pantoprazole Promotes Colonization of Helicobacter pylori SS1 in Mouse Stomachs
Author:
  • Shuo Yan 1,2

    Shuo Yan

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Xue Li 1,2

    Xue Li

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Chao Wang 1,2

    Chao Wang

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Jiali Xu 1,2

    Jiali Xu

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Yu Cheng 1,2

    Yu Cheng

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Liping Zhang 1,2

    Liping Zhang

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Lei Su 1,2

    Lei Su

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Jianan Gong 1,2

    Jianan Gong

    National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
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Affiliation:

1.National Center of Technology Innovation for Animal Model, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences &2.Peking Union Medical College

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    摘要:

    目的 寻找提高幽门螺旋杆菌(Helicobacter Pylori, Hp)在小鼠胃部定植效率的方法。明确质子泵抑制剂(Proton pump inhibitor; PPI)泮托拉唑是否能够作为定植辅助剂促进Hp在小鼠胃部定植,为建立Hp感染小鼠模型奠定基础。方法 建立幽门螺旋杆菌悉尼株(Sydney Strain 1, SS1)固体平板和液体培养体系并进行鉴定;比较不同剂量泮托拉唑对小鼠胃酸分泌的抑制效果;利用快速脲酶检测实验、细菌平板培养和TaqMan qPCR分析单独接种Hp或联合泮托拉唑预处理对Hp定植效率的影响。结果 质子泵抑制剂预处理能够抑制胃酸分泌在一定程度上促进Hp在小鼠胃部定植。结论 质子泵抑制剂能够作为定植辅助剂提高Hp感染小鼠模型的构建效率。

    Abstract:

    Objective To explore methods for improving the colonization efficiency of Helicobacter pylori (Hp) in the mouse stomach and to determine whether the proton pump inhibitor (PPI) pantoprazole can act as a colonization adjuvant to enhance Hp colonization. This study aims to provide an effective tool for establishing an Hp infection mouse model. Methods The Hp Sydney strain (SS1) was introduced, and solid plate and liquid culture systems were established and identified. The effects of different doses of pantoprazole on gastric acid secretion in mice were compared. The impact of Hp inoculation alone or combined with pantoprazole pretreatment on Hp colonization efficiency was analyzed using rapid urease tests, bacterial plate cultures, and TaqMan qPCR. Results Proton pump inhibitor pretreatment inhibited gastric acid secretion and, to some extent, promoted Hp colonization in the mouse stomach. Conclusion Proton pump inhibitors can serve as colonization adjuvants to enhance the efficiency of constructing Hp infection mouse models.

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历史
  • 收稿日期:2024-12-03
  • 最后修改日期:2024-12-19
  • 录用日期:2025-02-18
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