【】 Experimental animal facilities play a very important role in hospital scientific research work. Their construction standards and specifications, design principles and functional layout, equipment configuration and environmental control, as well as operation management, quality control, safety management, personnel training and other aspects should all follow unified standards and requirements. However, due to the complex and systematic nature of the construction of experimental animal facilities, each research institution and hospital has significant differences in the design concept, purpose, geographical location, construction requirements, personnel composition and other aspects of the facilities. In the specific construction and operation practice of experimental animal facilities, the problems encountered are also different. This article discusses the construction and operation of experimental animal facilities at the Central China Branch of the National Cardiovascular Disease Center, summarizes the experience of our unit in the construction and operation of experimental animal facilities, and provides theoretical guidance and practical reference for the construction and operation of experimental animal facilities in the hospital system.